Original Article
Najat Jabbar Ahmed Berwary, Rand Maurice Aziz
J Adv Biotechnol Exp Ther. 2022; 6(1): 210-221.

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ABSTRACT
Polycystic ovary syndrome (PCOS) is a widespread endocrine-reproductive-metabolic condition with severe implications for females’ health. The role of four essential parameters on PCOS, including human leukocyte antigens (HLA) represented by the HLA-B*0702 allele, anti-follicle stimulating hormone (anti-FSH) antibodies, anti-luteinizing hormone (anti-LH) antibodies, and vitamin D3 was investigated. A total of 100 samples were collected from Kurdish women attended the maternity teaching hospital and some private hospitals/laboratories in Erbil City from October 2021 to January 2022. The samples were genotyped using a PCR-based technique with specific sequence primers. The levels of anti-FSH and anti-LH antibodies were determined using an enzyme-linked immunosorbent assay (ELISA), while vitamin D3 levels were measured by an electrochemiluminescence (ECL) test on Cobas e411 immunoassay system. The odds ratio (OR) of 2.167 at a 95% confidence interval (CI) of 0.8167 to 6.330, indicated an essential link between the HLA-B*0702 allele and a risk of PCOS. Anti-FSH and anti-LH antibodies were significantly greater in PCOS patients, notably infertile women, than in healthy controls. A significant positive linear correlation was observed between antibodies against FSH and LH in patients. Most patients had hypovitaminosis D3 with a significant difference compared to controls. The results indicate that the HLA-B*0702 allele is associated with PCOS susceptibility and could be used as an immunogenetic marker. Besides, it supports the idea that anti-FSH, and anti-LH antibodies are naturally presence antibodies in PCOS patients instead of signs for autoimmunity. It also suggests that women suffer from PCOS are more prone to develop vitamin D3 deficiency.

KEYWORDS
Anti-FSH; Anti-LH; HLA- genotyping; Polycystic ovary syndrome; Vitamin D3.