Original Article
Mulia Indah Chandra Pertiwi, Manik Retno Wahyunitisari, Rebekah Juniati Setiabudi, Agung D.W. Widodo, Nurul Wiqoyah, M. Vitanata Arifijanto
J Adv Biotechnol Exp Ther. 2023; 6(2): 350-358.
ABSTRACT
Uropathogenic Escherichia coli (UPEC) is a fundamental cause of nosocomial urinary tract infection (UTI). UPEC can form biofilms on the mucosa of the urinary bladder and the surface of urethral catheters, thus causing clinical problems. Therefore, the current study aimed at the potential of synbiotics to manage biofilm-associated UTIs by analyzing biofilm inhibition UPEC with cell-free supernatant (CFS) of synbiotics. Biofilm inhibition was accomplished by inoculating each microbial suspension into 96-well microplates on tryptic soy broth medium at 37°C for 48 h and a microtiter plate reader was used at 595 nm to read the OD value. The outcome (%) was calculated from the OD value of CFS-treated with UPEC. The result of this research was that each CFS of synbiotic treatment displayed significantly different (P<0.05) results and was able to inhibit UPEC biofilm. The highest percentage of biofilm inhibition of UPEC was shown in CFS 8 treatment with a value of 41.51 ± 0.687, where CFS of synbiotic from Lactobacillus rhamnosus bacteria with 2% inulin. The lowest percentage of biofilm inhibition UPEC was shown in CFS 1 treatment with a value of 36.56 ± 1.987, where CFS from Lactobacillus plantarum bacteria with 0% inulin. It could be concluded that the higher the concentration of inulin in the CFS of synbiotics, the higher the percentage value of biofilm inhibition on UPEC, which indicates the potentials to manage or prevent UPEC-induced biofilm infection.
KEYWORDS
Biofilm inhibition; Cell-free supernatant of synbiotic; Uropathogenic Escherichia coli.