Original article| Open access | J Adv Biotechnol Exp Ther. 2025; 8(2): 342-352|doi: 10.5455/jabet.2025.28

Phytase supplementation in Broilers: Influence on growth performance and physiological health

Abstract

Phosphorus is essential for broiler growth and bone development, but its bioavailability from plant-based feed is restricted by phytate binding. Phytase supplementation enhances nutrient utilization by breaking down phytate, improving phosphorus availability, growth performance, gut health, and reducing environmental phosphorus excretion. Thus, this study aimed to evaluate the impact of phytase enzyme supplementation on growth performance and hemato-biochemical markers as a potential alternative to antibiotic growth promoters in broilers. In this study, 300 Arbor Acres broiler chicks were randomly assigned to five treatment groups (T0–T4) with three replicates each. The control group (T0) received a basic diet (1–42 days), while experimental groups T1 supplemented with oxytetracycline and T2–T4 were supplemented with Phytase (Natuphos® E) from days 7–42. T1 received oxytetracycline (1000 mg/kg), while T2, T3, and T4 were supplemented with 500, 750, and 1000 FTU/kg of Phytase, respectively. Growth performance was assessed through body weight, feed intake, feed conversion ratio (FCR), and livability. Blood samples were analyzed for hematological parameters (CBC) and biochemical markers, including total protein, albumin, ALT, AST, cholesterol, triglycerides, LDL, and HDL. Results indicated significant improvements in body weight and FCR in phytase-supplemented groups, with T4 showing the best performance (body weight 2915.1 g, FCR 1.53). Phytase supplementation improved hematological and biochemical profiles in broilers, with 1000 FTU/kg showing the highest efficacy. These data suggest that phytase enhances growth, feed efficiency, and physiological parameters, making it a viable alternative to antibiotic growth promoters.

References

  • [2]Chowdhury EU, Morey A. Intelligent Packaging for Poultry Industry. J Appl Poult Res. 2019; 28:791–800.
  • [3]Tanni FY, Rahman Chowdhury MS, et al. Prevalence and antimicrobial resistance of extended spectrum beta-lactamase (ESBL) producing Klebsiella spp. in poultry meat. Heliyon. 2025;11: e41748.
  • [4]Raihan S, Mahmud N. Trade and Poverty Linkages A Case Study of the Poultry Industry in Bangladesh 2008.
  • [5]Division RPP and H. Good practices in planning and management of integrated commercial poultry production in South Asia 2003.
  • [6]Hussain J, Rabbani I, et al. An overview of poultry industry in Pakistan. Worlds Poult Sci J. 2015; 71:689–700.
  • [7]Kamruzzaman M, Islam S, et al. Financial and factor demand analysis of broiler production in Bangladesh. Heliyon. 2021;7: e07152.
  • [8]Gholami-Ahangaran M, Ahmadi-Dastgerdi A, et al. Thymol and carvacrol supplementation in poultry health and performance. Vet Med Sci. 2021; 8:267.
  • [9]Liza NA, Hossain H, et al. Molecular Epidemiology and Antimicrobial Resistance of Extended-Spectrum β-Lactamase (ESBL)-Producing Klebsiella pneumoniae in Retail Cattle Meat. Vet Med Int. 2024;3952504.
  • [10]Rahman MdM, Hossain H, et al. Molecular Characterization of Multidrug-Resistant and Extended-Spectrum β-Lactamases-Producing Salmonella enterica Serovars Enteritidis and Typhimurium Isolated from Raw Meat in Retail Markets. Antibiotics. 2024; 13:586.
  • [11]Haque MH, Sarker S, et al. Sustainable Antibiotic-Free Broiler Meat Production: Current Trends, Challenges, and Possibilities in a Developing Country Perspective. Biology (Basel) 2020; 9:411.
  • [12]Selaledi LA, Hassan ZM, et al. The Current Status of the Alternative Use to Antibiotics in Poultry Production: An African Perspective. Antibiotics (Basel) 2020; 9:1–18.
  • [13]Paintsil EK, Ofori LA, et al. Antimicrobial Usage in Commercial and Domestic Poultry Farming in Two Communities in the Ashanti Region of Ghana. Antibiotics. 2021; 10:800.
  • [14]Rahman M, Parvin M, et al. Effects of growth promoter and multivitamin-mineral premix supplementation on body weight gain in broiler chickens. J Bangladesh Agril Univ. 2013; 10:245–8.
  • [15]Peric L, Zikic D, et al. Application of alternative growth promoters in broiler production. Biotechnology in Animal Husbandry 2009; 25:387–97.
  • [16]Nhung NT, Chansiripornchai N, et al. Antimicrobial Resistance in Bacterial Poultry Pathogens: A Review. Front Vet Sci. 2017; 4:126.
  • [17]Manyi-Loh C, Mamphweli S, et al. Antibiotic Use in Agriculture and Its Consequential Resistance in Environmental Sources: Potential Public Health Implications. Molecules. 2018; 23:795.
  • [18]Hossain H, Nuradji H, et al. Impact of synbiotic on growth performance, histo-architectural modulation of lymphoid organ, hematology, blood biochemistry and humoral immune response in naked neck chicken. Trop Anim Health Prod. 2025; 57:1–16.
  • [19]Carvalho IT, Santos L. Antibiotics in the aquatic environments: A review of the European scenario. Environ Int. 2016; 94:736–57.
  • [20]Gonzalez Ronquillo M, Angeles Hernandez JC. Antibiotic and synthetic growth promoters in animal diets: Review of impact and analytical methods. Food Control. 2017; 72:255–67.
  • [21]Dibner JJ, Richards JD. Antibiotic growth promoters in agriculture: history and mode of action. Poult Sci. 2005; 84:634–43.
  • [22]Melaku M, Zhong R, et al. Butyric and Citric Acids and Their Salts in Poultry Nutrition: Effects on Gut Health and Intestinal Microbiota. Int J Mol Sci. 2021; 22:10392.
  • [23]Diarra MS, Malouin F. Antibiotics in Canadian poultry productions and anticipated alternatives. Front Microbiol. 2014;5.
  • [24]Khan RU, Naz S, et al. Chromium: Pharmacological applications in heat-stressed poultry. International Journal of Pharmacology. 2014; 10:213–7.
  • [25]El-Fateh M, Bilal M, et al. Effect of antibiotic growth promoters (AGPs) on feed conversion ratio (FCR) of broiler chickens: A meta-analysis. Poult Sci. 2024; 103:104472.
  • [26]Singh PK. Significance of phytic acid and supplemental phytase in chicken nutrition: A review. Worlds Poult Sci J. 2008; 64:553–80.
  • [27]Rizwanuddin S, Kumar V, et al. Microbial phytase: Their sources, production, and role in the enhancement of nutritional aspects of food and feed additives. J Agric Food Res. 2023; 12:100559.
  • [28]Haque N, Hossain A. Phytase: Their Biochemistry, Physiology and Application in Poultry. Int J Livestock Res. 2012; 2:30.
  • [29]Rahman M, Hossain H, et al. Selection of efficient broiler strain for productive performances and immunity under local farming system in Bangladesh. J Adv Biotechnol Exp Ther. 2025; 8:218.
  • [30]Cowieson AJ, Bedford MR. The effect of phytase and carbohydrase on ileal amino acid digestibility in monogastric diets: complimentary mode of action? Worlds Poult Sci J. 2009; 65:609–24.
  • [31]Derakhshan M, Ghasemian SO, et al. The effects of probiotic and phytase on growth performance, biochemical parameters and antioxidant capacity in broiler chickens. Vet Med Sci. 2023; 9:860–6.
  • [32]Nawaz H, Rasheed S, et al. Effects of different fat sources on performance parameters and carcass characteristics of broiler chicks. Indian J Anim Sci. 2015; 85:414–8.
  • [33]Wang W, Wang Z, Yang H, Cao Y, Zhu X. Effects of phytase supplementation on growth performance, slaughter performance, growth of internal organs and small intestine, and serum biochemical parameters of broilers. Open J Anim Sci. 2013; 03:236–41.
  • []Raquib A, Uddin A, et al. Seroprevalence of Mycoplasma gallisepticum infection in layer chickens of Bangladesh. Iraqi J Vet Sci. 2022; 36:9–13.

Article Info

Academic Editor

Md Nabiul Islam, PhD; Yamaguchi University , Japan
Received
18 March, 2025
Accepted
03 May, 2025
Published
12 May, 2025

Coresponding author

Md. Mahfujur Rahman, PhD; Department of Medicine, Sylhet Agricultural University, Bangladesh. Email: mahfuj.vetmed@sau.ac.bd

Cite this article

Imranuzzaman M, Hossain H, et al. Phytase supplementation in Broilers: Influence on growth performance and physiological health. J Adv Biotechnol Exp Ther. 2025; 8(2): 342-352.